Real-Time PCR and RT-PCR Service
Real-time PCR (qPCR) is an extremely sensitive and reproducible method to measure levels of gene expression in an organism. It has quickly grown in popularity, and has become a revolutionary technology having supplanted the traditional approaches. It is widely accepted as a “Gold Standard” for DNA, and RNA quantification.
Gene Scientific, a leading expert in real-time PCR technology, now offers a rapid and reliable quantitative PCR analysis of gene expression and gene copy determination, which is available for all known gene regardless of species. The full service option includes SYBR Green or TaqMan based detection, RNA/DNA extraction, Reverse Transcription, primer/probe design, Absolute Quantification, MicroRNA expression, and complete data analysis.
Service Description
- RNA/DNA extraction from samples (Tissues or Cells) using total RNA/DNA isolation kit and/or TRIzol method.
- RNA/DNA quantitation and quality assessment using NanoDrop spectrophotometer / gel electrophoresis.
- Design qPCR primers/probe using state of the art software and design parameters; Each primer/probe set is validated for specificity by running qPCR and/or dissociation curve analysis. Pre-designed SYBR Green or Taqman Assays can be used at your request.
- Set up and run the real-time RT-PCR reactions (SYBR Green or Taqman). High quality total RNA samples are reverse transcribed using the First Strand cDNA Synthesis Kit, and run in triplicate qPCR.
- Data analysis. The averaged value is normalized to 18S rRNA or reference genes selected by customers for each sample. To evaluate the results, a ∆∆Ct method is used to compare changes in gene expression between controls and treated samples.
- MicroRNA (miRNA) qPCR Services. Highly accurate and sensitive miRNA assays for your studies.
- Absolute Quantification qPCR Services. Send us your standard sample, and we will determine the gene copy number with preparation of the Standard Curve.
Data Delivery
Data analysis is performed using our real-time RT-PCR data analysis software, and data can be delivered via email. Each customer receives complete study report including detailed experimental procedures, primer/probe sequences, raw Ct values, and data analysis file with gene expression fold-change information. We will support you before and after services while delivering expert technical consulation.
Pricing / Request a Quote
Services |
Price |
Size |
|---|---|---|
RNA Isolation, and Assessment |
$75.00 |
1 Sample |
Reverse Transcription (cDNA Synthesis) |
$18.00 |
1 Sample |
qPCR Assay Design, and Validation |
$35.00 |
1 Gene |
Real time PCR (Triplicate) |
$18.00 |
Per Sample per gene |
Contact us: info@genescientific.com, or fill the form at the bottom.
The highest standard and the lowest price guaranteed.
Why Gene Scientific?
Save time, save money with reliable results.
Let qPCR experts do the experiments for you.
Gene Scientific is committed to offer high quality service for scientific research in biotech industry and academia. For any question, please contact us: info@genescientific.com.
Quotation Form