AccuAmp SYBR Green qPCR Master Mixes

Benefits

• High PCR specificity for use with numerous different targets and templates.
• Outstanding sensitivity with early Ct detection.
• High amplification efficiency over a wide dynamic range.
• Convenient 2x master mix and optimized protocols

Description

AccuAmp SYBR Green qPCR Master Mixes are 2X concentrated, ready-to-use reaction cocktails that contain all components, except primers and template, for real-time quantative PCR. The master mixes provide highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using SYBR Green I. Since SYBR Green dye binds to all double-stranded DNA, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp SYBR Green qPCR Master Mixes promote highly specific annealing of primers to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.

Real-time PCR Instrument Compatibility

Different Real-time PCR instruments employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match appropriate qPCR reagent to your specific instrument according to the table below. 

Performance Data

A. High Sensitivity and Amplification efficiency Over a wild dynamic Range Detection of human GAPDH using AccuAmp SYBR Green qPCR Master Mix
B. Comparison Data: Higher Sensitivity and Lower Ct Detection of human RPL13A with the same serial dilutions of cDNA
Gene Scientific		Major Supplier A
C. Comparison Data: Higher Specificity
Gene Scientific		Major Supplier A