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AccuAmp Probe qPCR Master Mix/Low ROX

Cat. No.

Size

Price

Quantity

11002-100

100 x 25-µl reactions (1.25 ml)

$69.00

11002-400

400 x 25-µl reactions (4 x 1.25 ml)

$209.00

11002-01k

1000 x 25-µl reactions (12.5 ml)

$479.00

11002-02k

2000 x 25-µl reactions (25 ml)

$949.00

 

Benefits

Description

AccuAmp Probe qPCR Master Mix/ Low ROX is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe, and template, for real-time quantative PCR on Applied Biosystems 7500, 7500 Fast, ViiA 7, Stratagene Mx3000P, Mx3005P or Mx4000 instruments. This master mix provides highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using sequence-specific probe such as Taqman and other dual-labeled probes. For accurate Real-time PCR, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Probe qPCR Master Mix/ Low ROX promotes highly specific annealing of primers and probes to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.

Compatible Instrument

Storage

AccuAmp Probe qPCR Master Mix/ Low ROX is stable for 1 year when stored at –20°C in a constant-temperature freezer, protected from light.  After thawing, mix thoroughly before using.

Quick-Reference Protocol

1. Thaw AccuAmp Probe qPCR Master Mix/Low ROX (2X), template, primers, and probe. Mix the individual solutions.

2. Prepare a reaction mix.

Component

Volume/Reaction

Final Concentration

AccuAmp Probe qPCR Master
Mix/Low ROX (2X)

12.5 µl

1X

Forward Primer

Variable

400 nM

Reverse Primer

Variable

400 nM

Probe

Variable

100-250 nM

Template DNA or cDNA

Variable

Up to 100 ng/reaction

Nuclease-free Water

Variable

 

Total Reaction Volume

25µl

 

 

3. Mix the reaction mix thoroughly. After sealing each reaction, centrifuge reaction tubes briefly to avoid bubbles.

4. Program your thermal cycler.

Step

Temperature &Time

Notes

PCR initial activation step

95°C, 10 min

Hot-start Taq DNA polymerase is activated by this heating step.

2-step cycling (40 cycles)

a. 95°C, 15 sec (Denaturation)
b. 60°C, 1 min (Annealing/Extension)

Collect fluorescence data at step b.

 

5. Place the PCR tubes or plates in the real-time cycler, and start the cycling program.