GENE SCIENTIFIC

banner2

Log in

Cart

My Cart: 0 Item(s)

 

Products

Services

 

AccuAmp Probe qPCR Master Mix/Fluorescein

Cat. No.

Size

Price

Quantity

11003-100

100 x 25-µl reactions (1.25 ml)

$69.00

11003-400

400 x 25-µl reactions (4 x 1.25 ml)

$209.00

11003-01k

1000 x 25-µl reactions (12.5 ml)

$479.00

11003-02k

2000 x 25-µl reactions (25 ml)

$949.00

 

Benefits

Description

AccuAmp Probe qPCR Master Mix/ Fluorescein is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe, and template, for real-time quantative PCR on BioRad iCyler iQ, iQ5, MyiQ, or MyiQ2 instruments. This master mix provides highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using sequence-specific probe such as Taqman and other dual-labeled probes. For accurate Real-time PCR, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Probe qPCR Master Mix/ Fluorescein promotes highly specific annealing of primers and probes to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.

Compatible Instrument

Storage

AccuAmp Probe qPCR Master Mix/ Fluorescein is stable for 1 year when stored at –20°C in a constant-temperature freezer, protected from light.  After thawing, mix thoroughly before using.

Quick-Reference Protocol

1. Thaw AccuAmp Probe qPCR Master Mix/Fluorescein (2X), template, primers, and probe. Mix the individual solutions.

2. Prepare a reaction mix.

Component

Volume/Reaction

Final Concentration

AccuAmp Probe qPCR Master
Mix/Fluorescein (2X)

12.5 µl

1X

Forward Primer

Variable

400 nM

Reverse Primer

Variable

400 nM

Probe

Variable

100-250 nM

Template DNA or cDNA

Variable

Up to 100 ng/reaction

Nuclease-free Water

Variable

 

Total Reaction Volume

25µl

 

 

3. Mix the reaction mix thoroughly. After sealing each reaction, centrifuge reaction tubes briefly to avoid bubbles.

4. Program your thermal cycler.

Step

Temperature &Time

Notes

PCR initial activation step

95°C, 10 min

Hot-start Taq DNA polymerase is activated by this heating step.

2-step cycling (40 cycles)

a. 95°C, 15 sec (Denaturation)
b. 60°C, 1 min (Annealing/Extension)

Collect fluorescence data at step b.

 

5. Place the PCR tubes or plates in the real-time cycler, and start the cycling program.